Patents

StimuCell®: A Method and Electric Stimulation System for High Throughput Cellular Programming

Inventors:

V S N Sitaramgupta V, Nitu Bhaskar, Sudarshan Jagannathan, Asish Kumar Panda, Bikramjit Basu, and Hardik J. Pandya.

Abstract:

Electrical stimulation has successfully enhanced the functional restoration of tissues, bones during pre-clinical studies. Electrical stimulation enhances the production of proteins and cellular activities. Recent studies indicated that different electrical stimulation signals are being studied to elucidate its behaviour on the cells and tissues for treating chronic diseases. The cellular osteogenic responses and mineralization influenced by novel scaffolds or stimulation regimes are being evaluated by the in vitro model. To characterize cellular osteogenic responses to different stimulations, it is required to prepare samples on different well plates and provide different stimulation patterns to each well simultaneously for high throughput. Though there exist multiple commercial systems, either they are expensive or not flexible to use. This invention disclosure details the development of an affordable, compact electrical stimulation system that is compatible with 6, 24,48, and 96 well plates. The universal electronic circuit can detect the well plate connected to it and regulate different patterns on each row of the well plate. The parameters of the input stimulation signals and the corresponding wells in the well plate can be selected and controlled using a GUI installable on a Windows laptop or PC.  

Status:

Application Filed (June 08, 2021)

Indian Patent Application number:

202141025521 TEMP/E1/28678/2021- CHE

Device and Method for Rapid Detection of SARS-CoV Antigens and its Variants-of-Interest

Inventors:

Hardik J Pandya, Anju Joshi, Anil Vishnu G. K., Smitha P. K., and Manjula Das.

Abstract:

Most of the protein-protein interactions used in immunoassays involve a reaction between the target protein or antigen and its antibodies. Such immunoassays form the core of rapid antigen/diagnostic tests for viral infections such as the recent SARS-CoV pandemic. The major issues faced while designing an immunoassay include 1) Choosing specific and antibodies/antibody mimics for linking with the target ligand 2) Avoiding cases of nonspecific linkages and cross reactivity 3) Sensitive detection of antigen-antibody complex formation 4) Careful standardization of different physical and chemical parameters of the reaction surroundings and to reduce matrix effect. The scope for increasing the sensitivity, speed, and throughput of this technique demands more and more developments revolving around the principle of protein-protein interactions. While existing rapid detection label-free technologies have focused on lateral flow assays and electrochemical sensing, electrical impedance sensing presents itself as an easy-to-use and sensitive modality for detecting antigen-antibody binding events. This invention relates to the development and validation of such a device and methodology for high-throughput and sensitive detection of protein-protein interactions through antigen-antibody binding detection by electrical sensing for detecting SARS-CoV antigens and their variants through the detection of both the spike and nucleocapsid protein. Multiplexed microchips fabricated on industry-compatible ENIG and/or soft gold finish printed circuit board (PCB) are chemically modified for enhanced antibody immobilization and highly sensitive antigen detection. The microchips can be interfaced in a modular manner with either commercially available compact impedance sensing modules or custom electronic modules designed for specific applications. The assay has a limit of detection of 40 pg and can detect antigens with microlitre (20 – 40 mL) levels of analyte samples.

Status:

Application Filed

Indian Patent Application number:

202241039126 (July 07, 2022)

An apparatus for attaching a camera to a microscope

Inventors:

Hardik J Pandya, Aswin S, Hari R S, Akhil M, and Arjun B S.

Abstract:

The invention involves the design of a device that couples the camera to any microscope eyepiece, thereby transforming the microscope into a digital microscope. The device placed on the microscope eyepiece will align such that the optical axis of the camera and the microscope eyepiece become collinear. A rotatable ring on the device tightens the cantilever gripping elements and fastens the attachment module on the eyepiece.

Status:

Application Filed

Indian Design Patent Application number:

367940-001

A Reusable Multiangle Intradermal Drug Delivery Device

Inventors:

Hardik J Pandya, Arjun B S, Ajay Krishnan A, and Pushkraj Anil Janwadkar.

Abstract:

The invention involves a drug delivery device that can deliver fluids intradermally. The device that can be used with one hand is an injection device that can enable the patient to self-administer the drug with the press of a trigger. Alternatively, a second person can also use the device to administer the drug to a patient or the person who has to take the drug. The chamber within the device stores the container or syringe with the drug and mechanisms to push the syringe needle intradermally. The device consists of a mechanism that, on actuation, inserts the syringe needle tip to an intradermal depth, followed by the injection of the drug volume. The mechanism also retracts the needle after the injection automatically. The syringe can be taken out and disposed of post-injection, while a new syringe can be placed inside the device for reuse. The device also has the provision to adjust the angle at which the injection is carried out without affecting the depth of drug delivery. The base of the device can be firmly held against the skin before actuating the mechanism.

Status:

Application Filed

Indian Design Patent Application number:

202241033770

Reusable drug delivery device

Inventors:

Hardik J Pandya, Arjun B S, Ajay Krishnan A, Adithya Kumar, and Paramesh H.

Abstract:

Examples of a reusable drug delivery device and a method of using the reusable drug delivery device for delivering a drug to a patient are described. The reusable drug delivery device may include a hollow top casing including a first end with a safety and a second end. The hollo top casing encloses an injection mechanism slider, an injection mechanism holder, an injection spring, and a button spring. The reusable drug delivery device may further include a hollow bottom casing that encloses a retraction mechanism holder, a needle retraction spring, and a needle retractor. The reusable drug delivery device further includes a syringe unit comprising a syringe and a syringe holder.

Status:

Application Filed

Indian Patent Application number:

202241018326 (June 6, 2022)

A multimodal intraoperative probe for breast cancer margin assessment and methods thereof

Inventors:

Hardik J Pandya, Arif Mohd. Kamal, Uttam M. Pal, Arjun B. S., and Manu K. S.

Abstract:

The invention involves an intraoperative probe that can differentiate cancer margin during breast cancer resection. The probe can increase the extent of resection and the probability of attaining complete cancer resection, thus improving survival rates, better cosmetic outcomes, and the quality of life among the patients after surgery. With real-time results, the probe can aid in intraoperative decision-making. The probe and associated electronics are unique through the sensing modality they use. The probe integrates multiwavelength light sources, a monolithic photodiode with an inbuilt amplifier, a touch sensor switch, and an LED indicator unit. The probe is used for optical characterization of breast tissues for cancer delineation by measuring the different chromophores of the breast tissue with a high penetration depth, thereby attaining the best accurate results in thicker tissues. An indication of cancer boundary can be obtained using the data obtained from the probe and indicated on the laptop having a graphical user interface (GUI) module for assisting the surgeon in deciding on cancer margin assessment. Another modality is micromachined ultrasound transducers (MUTs) that will be incorporated with the optical modality to make it a multimodal probe to augment the cancer margin assessment.

Status:

Application Filed

Indian Patent Application number:

202241012649 (March 15, 2022)

An in-vivo, intraoperative probe for brain tumour margin delineation and methods thereof

Inventors:

Hardik J Pandya, Arjun B S, Anil Vishnu G. K., Gokul A M, Arun Baby, Shilpa Rao, Vikas V, Manish Beniwal and Anita Mahadevan.

Abstract:

The invention involves an intra-operative, in-vivo probe that can delineate tumour margin during brain tumour resection. The probe can increase the extent of resection and the probability of attaining gross tumour resection, thus leading to improved survival rates, as well as quality of life among the patients after surgical intervention. It also can address issues such as brain plasticity thereby enhancing the accuracy of brain tumour resection. With real-time results, the probe can aid in intra-operative decision-making. The probe, associated electronics and interface units are unique by means of the sensing modality they use. The probe is integrated with biochips or sensors or microarrays that are fabricated using technologies like MEMS or nanoimprint or screen printing or any of the other lithography-based processes or technology. These sensors can be multifunctional and thereby measure multiple parameters based on different principles for attaining the best accurate results. The probe design is such that it can reach out to complex surfaces and can be operated manually by hand as well as robotically. A virtual representation of tumour margin can be obtained using the data obtained from the probe and will be displayed on the interface unit for assisting the surgeon in resection.

Status:

Complete Application Filed

Patent Application number:

Indian: 202041022728 (May 30, 2020)

PCT: PCT/IB2021/055027 (June 08, 2021)

A handheld diagnostic tool for grading stenosis in pediatric upper airway and methods for characterizing the same.

Inventors:

Hardik J Pandya, Alekya B, Bhushan V, V S N Sitaram Gupta V, Arjun B S, Kevin Abhishek, S Siddesh Shenoy, Sanjay Rao and Mayur Bhuva.

Abstract:

The invention pertains to a sensor integrated diagnostic tool for management of pathological airway in pediatric population. The tool can be used for characterizing the tracheal obstruction, such as stenosis, of varying grades and anomaly types. The tool is a combination of an array of flow sensors and an unfurling actuator with radial arms that houses tactile sensors at the tip of the arm. The flow sensor array yields airflow patterns across various segments of the tracheobronchial tree and the unfurling actuator measures patency with tactile sensors capable of distinguishing between malacic and healthy tissue. The unfurler has radial arms that remain coiled inside the cylindrical casing at the distal tip. Upon actuation, the arms uncoil radially outward. The movement of the radial arms causes the tactile sensors to contact the tracheal walls and gives a measure of the tracheal diameter.

Status:

Filed

Indian Provisional Patent Application number:

TEMP/E-1/30202/2020-CHE

Date of Filing:

26th June 2020

International application number:

PCT/IB2021/054690

Date of Filing:

28th May 2021

Apparatus for High-Throughput Rapid Antibiotic Susceptibility Testing and Methods Thereof.

Inventors:

Hardik J Pandya, Anil Vishnu G K, Bhagaban Behera, Alekya B, Arun Baby, Saeed Rila B C, Arjun B S, Midhun C Kachappilly, Prathik BH, Dipshikha Chakravortty and Nagasuma Chandra.

Abstract:

The present disclosure provides a system for rapid antibiotic susceptibility testing. The system comprises portable electronic platform integrated with a cartridge containing a microfluidic chip, interdigitated electrodes, and microheaters for rapid, real-time, and label-free antibiotic susceptibility testing based on electrical sensing modality. A portable impedance analyser with a port is configured for holding the cartridge containing the microfluidic chip for AST testing.

Status:

Filed

Patent Application number:

TEMP/E-1/26941/2020-CHE

Date of Filing:

10th June 2020

Smart Wearable Device for Continuous Monitoring of Body Temperature and Blood Oxygen Saturation.

Inventors:

Hardik J Pandya, Jagannathan Gopalakrishnan, Sonal Asthana, Vishnu Kurpad, Anil Vishnu G K, Midhun C. Kachappilly and Arjun B S.

Abstract:

A smart wearable device for monitoring health status is disclosed, comprising a temperature sensor and a blood oxygen saturation (SpO2) sensor. The temperature sensor senses temperature of the user whereas the SpO2 sensor senses blood oxygen saturation and heart rate of the user. The device includes a control unit that receives at least one of temperature, heart rate, and SpO2 of the user from the temperature sensor and SpO2 sensor. The control unit also determines whether at least one of temperature, heart rate, and SpO2 falls below respective threshold. When the at least one of temperature, heart rate, and SpO2 falls below the threshold, the control unit generates an alert signal to alert the nearest health services about the health status of the user.

Patent Application number:

TEMP/E-1/29841/2020-CHE

Date of Filing:

25th June 2020

Apparatus and System for Rapid Diagnosis of Malignant Lesions from Biopsy Tissues for Use Inside the Operation Room.

Inventors:

Hardik J Pandya, Anil Vishnu G K, Bhagaban Behera, Saeed Rila, Midhun C Kachappilly, Arun Baby and Annapoorni Rangarajan.

Abstract:

The present disclosure provides a system for rapid diagnosis of malignant lesions from biopsy tissue. The system comprises table-top platform integrated with microsensors and electronic modules for label-free and rapid phenotyping of extracted biopsy tissues using multiple modalities for a quick diagnosis, and to identify the margin for use inside the operation room. A multiple logistic regression model takes all the physical parameters measured as input variables to accurately predict the diagnosis. The electrical resistivity, thermal conductivity and mechanical stiffness of the extracted biopsy tissues are measured and used for performing the diagnostic delineation. The computations are performed on-board and the entire system can be controlled and actuated using a smart phone

Patent Application number:

TEMP/E1/33093/2020-CHE

Date of Filing:

30th May 2020

Non-invasive test kit for rapid detection and screening infectious disease in individuals

Inventors:

Hardik J Pandya, Jagannathan Gopalkrishnan, Anil Vishnu G K, Midhun C. Kachappilly, Sudarshan Jagannathan, Akhil Mohan and Uttam M. Pal.

Indian patent application filing no.:

202041022006

Date of Filing:

May 26, 2020

Portable Multi-Parameter Cancer Diagnostic Device

Inventors:

Jaydev P. Desai, Hardik J Pandya and Kihan Park.

Provisional US Patent Application No.:

62/112867

Date of Filing:

6th February 2015.

An Improved Micro-heater in Silicon Substrate using Trench Formation, Backfill and CMP

Inventors:

Sudhir Chandra and Hardik J Pandya.

Indian patent application filing no.:

1619/DEL/2012

Date of Filing:

29th May 2012

MEMS Based Energy Efficient Sensors on Oxide Platform in Silicon Substrate for Detection of Volatile Organic Compounds and Gases

Inventors:

Sudhir Chandra and Hardik J Pandya.

Indian patent application filing no.:

1802/DEL/2012

Date of Filing:

12th June 2012